Small Animal Imaging Core

About Us

The Small Animal Imaging Core provides investigators with access to radiology imaging equipment and specialized high end computers and software for research that involves animal models of health and disease. Its function is similar to radiology departments in the clinical setting. Staffed by highly experienced research imaging specialists and technologists, the core provides images to investigators and facilitates image processing and interpretation via collaboration with the Translational Biomedical Imaging Laboratory, the Optical Imaging Core and individual research laboratories.  The equipment is typical of instrumentation found radiology department with the addition of special optical based techniques useful for imaging very small subjects: Bioluminescence/Fluorescence imaging, Micro-CT, 11.7 Tesla small bore MRI, High-Res plain film X-ray, Micro-Spect-CT.

How We Support

The facility is designed to provide not only imaging technologies, but also guidance in terms of imaging protocols. One of the core's major capabilities is handling different kinds of research projects including working with small rodents with or without imaging equipment. The core is open to academic investigators and commercial/non-commercial organizations.

Please review the general Core Use Policy.

Fee Structure

The following internal prices are for Principal Investigators at CHLA and USC; rates are subject to change in the future.

2022 SAIC Line Item Costs

ServiceCost
MRI Imaging$220.00/hr
MRI-Overnight (5 p.m. thru 9 a.m.)$450
uCT Imaging$165.00/hr
BIoLuminescence$145.00/hr
Plain Film Radiograph$11.00/hr
Intra-Cranial Xenografts$82.50/per mouse
Animal Handling, Treatments Biological Tissue Harvesting$38.50/hr
Animal HusbandrySTD-$0.52, Cage per day
ID Mice-$0.57, Cage per day
Rats-$2.00, Cage per day
Animal Purchase$110.00, varies by strain and type
Hemi-Splenic Xenographts (survival surgery)$82.50
Beetle Luciferin (BLI contrast)$460.00/gr, can vary
Data Reconstruction$44.00/hr
Data Processing$88.00/hr
Software Training$88.00

2022 SAIC Full Project Costs

ServiceCost
Professional Time$110.00/hr
Technologist Time$38.50/hr
Administrative Time$55.00/hr
IACUC Protocol Submission$5,500.00
MaterialsPrice on Request
Other Imaging Contrast MaterialsPrice on Request

 

  • Services
  • Equipment
  • Publications
  • Imaging Data Analysis

The Small Animal Imaging core provides basic images to investigators as well as image data collection, reconstruction, and storage. Image processing and interpretation require collaboration with the Radiology Department.

In addition, the Core provides project management, precise intracranial xenographts, fixed and non-fixed biological sample harvesting, complicated perfusions, technical training and consultations, and access to advanced computer systems.

CoreConnect

This facility utilizes CoreConnect, a web-based core management system that supports the centralization of services and equipment scheduling, billing and usage tracking. Use of the new system is required for all core users, core leaders and core staff.

Learn more about CoreConnect

This Core participates in the CHLA Core Pilot Program. To learn more click here.

CHLA welcomes external users to utilize our Core facilities. Please contact Gevorg Karapetyan at gkarapetyan@chla.usc.ed.

uMRI HiRes.jpg11.7 T BioSpec uMRI (Bruker) 2020 - A multipurpose high field MR scanner for both magnetic resonance imaging(MRI) and magnetic resonance spectroscopy(MRS) for preclinical, pharmaceutical, and fundamental research. The instrument is based on the AVANCE NEO MRI scanner architecture, consisting of 11.7T, 11cm Bore US/R magnet, Faraday RF-Shielding Cabinet, Main Gradient and Shim, MRI RF Coil, MRI Cryo Coil, 1H Transmit and Receive Channel.

 

 

 


 

CHLA-Research-Imaging-2264-275.jpgIVIS 200 Bioluminescence/Fluorescence (Perkin-Elmer, former Xenogen) 2013 - in vivo imaging technology platform, that allows non-invasive visualization and tracking of cellular and genetic activity within a living organism in real time.  This equipment is work-horse with high throughput capacity. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using Digital Mouse Atlas.

 

 

 

 

 

 


 

Faxitron MXCHLA-Research-Imaging-2265-275.jpg-20- is the industry standard in cabinet X-ray systems for point-of-care specimen/small rodent radiography, specified in bone studies and implants.

 

 

 


 

CHLA-Research-Imaging-2266-275.jpgSkyScan1172 HighRes uCT (Bruker) - High-resolution desktop X-ray  uCT system for small samples. The flexible geometry of the scanner is particularly advantageous over intermediate resolution levels.  Good for biological tissue structural studies.

 

 


 

How to cite the Core in publications:

The _______ (select services: MRI imaging studies, MRS studies, CT imaging studies, In Vivo Fluorescence Imaging Studies, In Vivo Bioluminescence Imaging Studies, or analysis of the images above only) was performed in the Small Animal Imaging Core at The Saban Research Institute, Children's Hospital Los Angeles.

Publications

Sims JD, Hwang JY, Wagner S, Alonso-Valenteen F, Hanson C, Taguiam JM, Polo R, Harutyunyan I, Karapetyan G, Sorasaenee K, Ibrahim A, Marban E, Moats R, Gray  HB, Gross Z, Medina-Kauwe LK. A corrole nanobiologic elicits tissue-activated MRI contrast enhancement and tumor-targeted toxicity. J Control Release. 2015 Nov 10;217:92-101. doi: 10.1016/j.jconrel.2015.08.046. Epub 2015 Aug 31. PubMed PMID: 26334483; PubMed Central PMCID: PMC4623877.

Bonne NX, Vitte J, Chareyre F, Karapetyan G, Khankaldyyan V, Tanaka K, Moats  RA, Giovannini M. An allograft mouse model for the study of hearing loss secondary to vestibular schwannoma growth. J Neurooncol. 2016 Aug;129(1):47-56. doi: 10.1007/s11060-016-2150-9. Epub 2016 May 13. PubMed PMID: 27177628.

Morshed RA, Gutova M, Juliano J, Barish ME, Hawkins-Daarud A, Oganesyan D, Vazgen K, Yang T, Annala A, Ahmed AU, Aboody KS, Swanson KR, Moats RA, Lesniak MS. Analysis of glioblastoma tumor coverage by oncolytic virus-loaded neural stem cells using MRI-based tracking and histological reconstruction. Cancer Gene Ther. 2015 Jan;22(1):55-61. doi: 10.1038/cgt.2014.72. Epub 2014 Dec 19. PubMed PMID: 25525033; PubMed Central PMCID: PMC4293243.

Gutova M, Frank JA, D'Apuzzo M, Khankaldyyan V, Gilchrist MM, Annala AJ, Metz MZ, Abramyants Y, Herrmann KA, Ghoda LY, Najbauer J, Brown CE, Blanchard MS,Lesniak MS, Kim SU, Barish ME, Aboody KS, Moats RA. Magnetic resonance imaging tracking of ferumoxytol-labeled human neural stem cells: studies leading to clinical use. Stem Cells Transl Med. 2013 Oct;2(10):766-75. doi: 10.5966/sctm.2013-0049. Epub 2013 Sep 6. PubMed PMID: 24014682; PubMed Central PMCID: PMC3785261.

Tang Y, Sharma P, Nelson MD, Simerly R, Moats RA. Automatic abdominal fat assessment in obese mice using a segmental shape model. J Magn Reson Imaging. 2011 Oct;34(4):866-73. doi: 10.1002/jmri.22690. Epub 2011 Jul 18. PubMed PMID: 21769982.

Tang Y, Lee S, Nelson MD Jr, Richard S, Moats RA. Adipose segmentation in small animals at 7T: a preliminary study. BMC Genomics. 2010 Dec 1;11 Suppl 3:S9. doi: 10.1186/1471-2164-11-S3-S9. PubMed PMID: 21143791; PubMed Central PMCID: PMC2999354.

The Research Imaging Core is equipped with a wide variety of commercial tool sets for imaging data analysis and visualization.

CTAn, CTVol - Bruker's data analysis and visualization tool suite

Analysis - Olympus's image analysis software

Amira - FEI's platform for 3D and 4D data visualization, processing and analysis

Fiji (ImageJ) - A NIH image processing tool

Lightwave 3D - NewTek's 3D computer graphics software

Imaris - 3D and 4D Real-Time Interactive Data Visualization Platform

Living image - In Vivo Imaging Software

Arivis 4D - Data visualization Platform

Analyze Direct – Image processing software

MATLAB – computing environment and proprietary programming

Visual Studio - Interactive developmental environment for programming