Rodent Metabolic Core

The Saban Rodent Metabolic Core offers access to state-of-the-art technology and high quality metabolic phenotyping services to basic science researchers studying diabetes, obesity and metabolism. Phenotyping experiments are conducted at CHLA by our highly trained and experienced core personnel using the most up to date, standardized techniques.

Services

  • Body composition (NMR)
  • Indirect calorimetry
  • Locomotor activity
  • Food intake
  • Glucose and insulin tolerance
  • Pyruvate tolerance
  • Leptin tolerance
  • Glucose CLAMP
  • Sample collection
  • Hormone and lipid assay
  • Core temperature
  • Consultation and training
  • TissueLyser

For Investigators

For more information about utilizing the Rodent Metabolic Lab, please contact Laura Gatewood at lgatewood@chla.usc.edu.

 

  • Fee Structure
  • Core Acknowledgment in Publications
Equipment/Service Fee
Body Composition $15
Metabolic Cages $800 (first run)- CHLA/USC
$400 (second run)- CHLA/USC
$1,200 (first run)- External Investigators
$800 (second run)- External Investigators
Glucose Tolerance $150/sample
Insulin Tolerance $150/sample
Pyruvate Tolerance $150/sample
Hyperinsulinemic, Euglycemic glucose CLAMP $300/sample- CHLA/USC
$400/sample- External Investigators
Plasma Hormone Analysis $100 service fee/plate + the cost of ELISA kit
Plasma Lipid Analysis $10/sample
Core Body Temperatures $5/sample
Tissue Collection $15-$25/sample
Equipment/Service
Body Composition | Fee: $15
Metabolic Cages | Fee: $800 (first run)- CHLA/USC | $400 (second run)- CHLA/USC | $1,200 (first run)- External Investigators | $800 (second run)- External Investigators
Glucose Tolerance | Fee: $150/sample
Insulin Tolerance | Fee: $150/sample
Pyruvate Tolerance | Fee: $150/sample
Hyperinsulinemic, Euglycemic glucose CLAMP | Fee: $300/sample- CHLA/USC | $400/sample- External Investigators
Plasma Hormone Analysis | Fee: $100 service fee/plate + the cost of ELISA kit
Plasma Lipid Analysis | Fee: $10/sample
Core Body Temperatures | Fee: $5/sample
Tissue Collection | Fee: $15-$25/sample

How to cite the Core in publications:

This work was performed with the support of the Rodent Metabolic Core of The Saban Research Institute at Children's Hospital Los Angeles.

Recent Publications:

Croizier, S., Park, S., Maillard, J., & Bouret, S. G. (2018). Central Dicer-miR-103/107 controls developmental switch of POMC progenitors into NPY neurons and impacts glucose homeostasis. ELife. 10.7554/eLife.40429

van der Klaauw, A. A., Croizier, S., Mendes de Oliveira, E., Stadler, L. K. J., Park, S., Kong, Y., … Bouret, S.G., Farooqi, I. S. (2019). Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance. Cell. https://doi.org/10.1016/j.cell.2018.12.009

Woodward NC, Crow AL, Zhang Y, Epstein S, Hartiala J, Johnson R, Kocalis H, Saffari A, Sankaranarayanan I, Akbari O, Ramanathan G, Araujo JA, Finch CE, Bouret SG, Sioutas C, Morgan TE, Allayee H. Exposure to Nanoscale Particulate Matter from Gestation to Adulthood Impairs Metabolic Homeostasis in Mice. Sci Rep. 2019 Feb 12;9(1):1816. doi: 10.1038/s41598-018-37704-2. PubMed PMID: 30755631; PubMed Central PMCID: PMC6372675.