Stem Cell Analytics Core

About Us

The Stem Cell Analytics Core is a centralized support facility for the entire research community whose primary mission is to facilitate stem cell research, innovation, and interactions to ensure the success of funded research projects.

How We Support

The Stem Cell Analytics Core offers support by providing human pluripotent cells (hPSC), hPSC culture training, and supplies management (including ordering, inventory, stocking). The Core also allows investigators to rent shared, well maintained, and fully equipped stem cell culture facilities. Additionally, the Core provides the following cell line validation services: mycoplasma and pluripotency testing and third party STR identification and genetic integrity testing (qPCR based test). We also provide access to shared equipment such as real time PCR machine, nucleofector, 96-well shuttle, fluorescence and dissection microscopes, and freezers.

CoreConnect

This facility utilizes CoreConnect, a web-based core management system that supports the centralization of services and equipment scheduling, billing and usage tracking. Use of the new system is required for all core users, core leaders and core staff.

Learn More about CoreConnect

This Core participates in the CHLA Core Pilot Program. To learn more click here.

  • Research Services and Equipment
  • Fee Structure
  • Stem Cell Analytics Core Policies
  • Publications

The Stem Cell Analytics Core offers cell distribution, biobanking and stem cell testing services, assisting both Children’s Hospital Los Angeles investigators and external investigators with their research.

The staff at the Stem Cell Analytics Core provides high quality human pluripotent stem cell lines (hPSC), hPSC culture training, mycoplasma testing, cell line authentication as well access to rent shared, meticulously maintained and fully equipped tissue culture facilities. Additionally, the Stem Cell Analytics Core can help investigators using the Core with supplies management, including ordering, stocking and keeping inventory, and they can provide tissue culture supplies, like pluripotent stem cell related reagents, at a discount through bulk purchasing.

The following equipment is available within the Stem Cell Analytics Core:

Amaxa Nucleofector II with or without Lonza 96-well shuttle

Amaxa-Nucleofactor.jpg Lonza-96-Well-Shuttle.jpg

Countess automated cell counter

Countess-Automated-Cell-Counter.jpg

 Nikon dissection microscopes

Nikon-Dissection-Microscope.jpg

 Liquid nitrogen and -80 freezer storage space

Liquid-Nitrogen-Freezer-(1).jpg Liquid-Nitrogen-Freezer-(2).jpg _80C-Freezer.jpg

QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks

1. QuantStudio 7 Flex Real-Time PCR System.jpg

TS100 Fluorescent microscope

TS100 Fluorescent microscope.jpg

This Core participates in the CHLA Core Pilot Program. To learn more click here.

CHLA welcomes external users to utilize our Core facilities. Please contact Narine Harutyunyan at NHarutyunyan@chla.usc.edu or 323-361-3665.

Note: Prices are subject to change in the future.

The following prices are for Principal Investigators at CHLA.

PDF download of fee structure

Services

Size

Price

Cells/Cell Services

WA01(H1)*,WA09(H9)*, Feeder Free 1 35mm dish $45
MEF base cultures available upon request 1 35mm dish $45
Each additional dish

 

$5/dish
Technical support

 

$37/hr
Frozen WA01(H1)* (~p55) 1 vial

$200

Frozen WA09(H9)* (~p30) 1 vial $350

Cell pellets

1 tube (1e6 cells) $45

Freezing cell lines

 

$10/vial
Maintenance PSC/iPSC cell line provided by researcher

 

$110/line/week
*Cell lines are available only to CHLA researchers with the appropriate agreement executed by WiCell

 

Equipment

QuantStudio™ 7 Flex Real-Time PCR System (fast 96-well, 384-well, TLDA card blocks)

 

$20/hour
Nucleofector II

 

$15/use
Nucleofector II + 96-well Shuttle

 

$25/use
Nikon TS100 Fluorescent tissue culture microscope**

 

$20/hour
**included in regular user's monthly charge or $100/month maximum

 

Services

Mycoplasma testing (needed for NIH grants)

 

$35/sample
Custom human iPSC reprogramming (includes generation of clonal iPSC lines for a patient blood sample provided by customer and Quality control assays)

 3 clones

$6,500
Pluripotency testing

 

$50/sample
Genetic testing by PCR via Stem Genomics***   $180/sample

STR authentication via University of Arizona (needed for NIH grants)***

 

$66/sample (if submitting genomic DNA)   $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)
**Free shipping to offer to all TSRI once per month

 

Facility Charges

Facility usage charge (1st hood and all equipment)

 

$150/month
Facility usage charge (each additional hood)

 

$75/month
Supplies (Plastic and Media)

 

At cost
LN2 storage

 

$5/box/month
Freezer Storage (-20 or -80C)

 

$5/box/month

Working with Human Pluripotent Stem Cells

All research involving human pluripotent stem cells must be reviewed and approved by the Children's Hospital Los Angeles Stem Cell Research Oversight (SCRO) Committee. Investigators requesting H1 or H9 embryonic stem cells will be also be required to sign a Material Transfer Agreement (MTA), termed a simple letter agreement (SLA) from WiCell, before receiving cells. Material Transfer Agreements are required for each cell line used.

For information or questions on SCRO policies and procedures prior to submitting a protocol application, please contact the Institutional Review Board at Children’s Hospital Los Angeles at hspp@chla.usc.edu.

For information and questions on material transfer or to get started on a Material Transfer Agreement, please contact the Office of Technology Commercialization team.

For general lab safety questions and information about lab safety policy, please reach out to labsafety@chla.usc.edu.