Stem Cell Analytics Core
About Us
The Stem Cell Analytics Core is a centralized support facility for the entire research community whose primary mission is to facilitate stem cell research, innovation, and interactions to ensure the success of funded research projects.
How We Support
The Stem Cell Analytics Core offers support by providing human pluripotent cells (hPSC), generating custom human induced pluripotent cells (iPSCs), hPSC culture training, and supplies management (including ordering, inventory, stocking). The Core also allows investigators to rent shared, well maintained, and fully equipped stem cell culture facilities. Additionally, the Core provides the following cell line validation services: mycoplasma and pluripotency testing and third party STR identification and genetic integrity testing (qPCR based test). We also provide access to shared equipment such as real time PCR machine, nucleofector, 96-well shuttle, fluorescence and dissection microscopes, and freezers.
Please review the general Core Use Policy.
CoreConnect
This facility utilizes CoreConnect, a web-based core management system that supports the centralization of services and equipment scheduling, billing and usage tracking. Use of the new system is required for all core users, core leaders and core staff.
Learn More about CoreConnect
This Core participates in the CHLA Core Pilot Program. To learn more click here.
Fee Structure
CHLA welcomes external users to utilize our Core facilities. Please contact Narine Harutyunyan at NHarutyunyan@chla.usc.edu or 323-361-3665.
The following internal prices are for Principal Investigators at CHLA and USC; rates are subject to change in the future.
Stem Cell Analytics Core Fee Structure as of November 2022
| Services | Size | Price |
|---|---|---|
| Cells/Cell Services | ||
| WA01(H1)*,WA09(H9)*, Feeder Free; 2nd dish and beyond | 1 35mm dish | $90.00 |
| MEF base cultures available upon request | 1 35mm dish | $90.00 |
| Each additional dish | $10/dish | |
| Frozen WA01(H1)* (~p55); frozen vial or 1st dish | 1 vial or 1 35mm dish | $200.00 |
| Frozen WA09(H9)* (~p30); frozen vial or 1st dish | 1 vial or 1 35mm dish | $200.00 |
| iPSC Control Cell Line C275.3* (~p19) | 1 vial or 1 35mm dish | $200.00 |
| iPSC Control Cell Line CHLA-H19M | 1 vial or 1 35mm dish | $200.00 |
| Technical support | $40/hr | |
| Training course - pluripotent stem cells (1st person) | $485.00 | |
| Training course - pluripotent stem cells (additional person) | $205.00 | |
| Freezing cell lines | $10/vial | |
| Maintenance PSC/iPSC cell line provided by researcher | $135/line/week | |
| *Cell lines are available only to CHLA researchers with the appropriate agreement executed by WiCell or FUJIFILM CDI. | ||
| Equipment | ||
| QuantStudio™ 7 Flex Real-Time PCR System (fast 96-well, 384-well, TLDA card blocks) | $20/hour | |
| Nucleofector II | $15/use | |
| Nucleofector II + 96-well Shuttle | $25/use | |
| Nikon TS100 Fluorescent tissue culture microscope** | $20/hour | |
| **Included in regular user's monthly charge or $100/month maximum | ||
| Services | ||
| Mycoplasma testing (needed for NIH grants) qPCR based | $40/sample | |
| Custom human iPSC reprogramming (includes generation of clonal iPSC lines for a patient blood sample provided by customer and Quality control assays) | 3 clones | $7,000.00 |
| Pluripotency testing | $50/sample | |
| Genetic SCNA testing by PCR via Stem Genomics | $180/sample | |
| STR authentication via University of Arizona (needed for NIH grants)*** | • $66/sample (if submitting genomic DNA) • $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction) | |
| ***Free shipping to offer to all TSRI once per month | ||
| Facility Charges | ||
| Facility usage charge (1st hood and all equipment) | $170.00 | |
| Facility usage charge (each additional hood) | $85.00 | |
| Supplies (Plastic and Media) | At cost | |
| LN2 storage | $5/box/month | |
| Freezer Storage (-20 or -80C) | $5/box/month | |
- Research Services and Equipment
- Stem Cell Analytics Core Policies
- Publications
The Stem Cell Analytics Core offers cell distribution, biobanking and stem cell testing services, assisting both Children’s Hospital Los Angeles investigators and external investigators with their research.
The Stem Cell Analytics Core offers:
- Human pluripotent stem cell line (hPSC) distribution: WA01,WA09
- Custom human iPSC reprogramming from blood or fibroblasts
- Maintenance of investigator iPSC lines
- hPSC culture training (feeder or feeder free)
- General tissue culture training
- Mycoplasma testing
- Pluripotency testing
- Genetic testing by PCR via Stem Genomics
- STR cell line authentication via University of Arizona
- Rental of fully equipped tissue culture facilities
Stem Cell Analytics Core can help investigators using the Core with supplies management, including ordering, stocking and keeping inventory, and they can provide tissue culture supplies, like pluripotent stem cell related reagents, at a discount through bulk purchasing.
The following equipment is available within the Stem Cell Analytics Core:
- QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks
- Nucleofector II with or without Lonza 96-well shuttle
- Dissection microscopes
- TS100 Fluorescent microscope
- Countess automated cell counter
- Liquid nitrogen and -80 freezer storage space
QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks
Amaxa Nucleofector II with or without Lonza 96-well shuttle
Nikon dissection microscopes
TS100 Fluorescent microscope
Countess automated cell counter
Liquid nitrogen and -80 freezer storage space
This Core participates in the CHLA Core Pilot Program. To learn more click here.
Working with Human Pluripotent Stem Cells
All research involving human pluripotent stem cells must be reviewed and approved by the Children's Hospital Los Angeles Stem Cell Research Oversight (SCRO) Committee. Investigators requesting H1 or H9 embryonic stem cells will be also be required to sign a Material Transfer Agreement (MTA), termed a simple letter agreement (SLA) from WiCell, before receiving cells. Material Transfer Agreements are required for each cell line used.
For information or questions on SCRO policies and procedures prior to submitting a protocol application, please contact the Institutional Review Board at Children’s Hospital Los Angeles at hspp@chla.usc.edu.
For information and questions on material transfer or to get started on a Material Transfer Agreement, please contact the Office of Technology Commercialization team.
For general lab safety questions and information about lab safety policy, please reach out to labsafety@chla.usc.edu.
Please review the general Core Use Policy.
To view the SCAC Standard Operating Procedure, please click here.
How to cite the Core in publications:
This work was assisted by the Stem Cell Analytics Core at The Saban Research Institute, Children's Hospital Los Angeles.
Following any publication that cites the Core, please inform the Core at stemcellcore@chla.usc.edu
Recent publications include:
Core Reagent Inventory
Select Cores offer reagents as part of their purchasable services to the CHLA research community. A searchable Core Reagent Inventory was created in support of the CHLA research community BenchResearchHelp initiative that connects researchers with reagents when in need of a small amount of a reagent, such as an antibody, to determine the efficacy in a particular experiment.
CHLA based investigators are welcomed to browse the Cores Reagent Inventory to access the complete product list for pricing and availability. The database will be updated on an annual basis. Contact the Core for more information.