Stem Cell Analytics Core

About Us

The Stem Cell Analytics Core is a centralized support facility for the entire research community whose primary mission is to facilitate stem cell research, innovation, and interactions to ensure the success of funded research projects.

How We Support

The Stem Cell Analytics Core offers support by providing human pluripotent cells (hPSC), generating custom human induced pluripotent cells (iPSCs), hPSC culture training, and supplies management (including ordering, inventory, stocking). The Core also allows investigators to rent shared, well maintained, and fully equipped stem cell culture facilities. Additionally, the Core provides the following cell line validation services: mycoplasma and pluripotency testing and third party STR identification and genetic integrity testing (qPCR based test). We also provide access to shared equipment such as real time PCR machine, nucleofector, 96-well shuttle, fluorescence and dissection microscopes, and freezers.

Please review the general Core Use Policy.

CoreConnect

This facility utilizes CoreConnect, a web-based core management system that supports the centralization of services and equipment scheduling, billing and usage tracking. Use of the new system is required for all core users, core leaders and core staff.

Learn More about CoreConnect

This Core participates in the CHLA Core Pilot Program. To learn more click here.

Fee Structure

CHLA welcomes external users to utilize our Core facilities. Please contact Narine Harutyunyan at NHarutyunyan@chla.usc.edu or 323-361-3665.

The following internal prices are for Principal Investigators at CHLA and USC; rates are subject to change in the future.

PDF download of fee structure

Services Size Price
Cells/Cell Services
WA01(H1)*,WA09(H9)*, Feeder Free 1 35mm dish $45
MEF base cultures available upon request 1 35mm dish $45
Each additional dish   $5/dish
Technical support   $37/hr
Frozen WA01(H1)* (~p55) 1 vial $200
Frozen WA09(H9)* (~p30) 1 vial $350
Cell pellets 1 tube (1e6 cells) $45
Freezing cell lines   $10/vial
Maintenance PSC/iPSC cell line provided by researcher   $110/line/week
*Cell lines are available only to CHLA researchers with the appropriate agreement executed by WiCell
 
Equipment
QuantStudio™ 7 Flex Real-Time PCR System (fast 96- well, 384-well, TLDA card blocks)   $20/hour
Nucleofector II   $15/use
Nucleofector II + 96-well Shuttle   $25/use
Nikon TS100 Fluorescent tissue culture microscope**   $20/hour
**Included in regular user's monthly charge or $100/month maximum
 
Services
Mycoplasma testing (needed for NIH grants)   $35/sample
Custom human iPSC reprogramming (includes generation of clonal iPSC lines for a patient blood sample provided by customer and Quality control assays) 3 clones $6,500
Pluripotency testing   $50/sample
Genetic testing by PCR via Stem Genomics   $180/sample
STR authentication via University of Arizona
(needed for NIH grants)***
  • $66/sample
(if submitting genomic DNA)
• $75.6/sample
(if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)
***Free shipping to offer to all TSRI once per month
 
Facility Charges
Facility usage charge (1st hood and all equipment)   $150/month
Facility usage charge (each additional hood)   $75/month
Supplies (Plastic and Media)   At cost
LN2 storage   $5/box/month
Freezer Storage (-20 or -80C)   $5/box/month

 

  • Research Services and Equipment
  • Stem Cell Analytics Core Policies
  • Publications

The Stem Cell Analytics Core offers cell distribution, biobanking and stem cell testing services, assisting both Children’s Hospital Los Angeles investigators and external investigators with their research.

The Stem Cell Analytics Core offers:

  • Human pluripotent stem cell line (hPSC) distribution: WA01,WA09
  • Custom human iPSC reprogramming from blood or fibroblasts
  • Maintenance of investigator iPSC lines
  • hPSC culture training (feeder or feeder free)
  • General tissue culture training
  • Mycoplasma testing
  • Pluripotency testing
  • Genetic testing by PCR via Stem Genomics
  • STR cell line authentication via University of Arizona
  • Rental of fully equipped tissue culture facilities

Stem Cell Analytics Core can help investigators using the Core with supplies management, including ordering, stocking and keeping inventory, and they can provide tissue culture supplies, like pluripotent stem cell related reagents, at a discount through bulk purchasing.

The following equipment is available within the Stem Cell Analytics Core:

  • QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks
  • Nucleofector II with or without Lonza 96-well shuttle
  • Dissection microscopes
  • TS100 Fluorescent microscope
  • Countess automated cell counter
  • Liquid nitrogen and -80 freezer storage space

QuantStudio™ 7 Flex Real-Time PCR System with fast 96-well, 384-well, TLDA card blocks

1. QuantStudio 7 Flex Real-Time PCR System.jpg

Amaxa Nucleofector II with or without Lonza 96-well shuttle

Amaxa-Nucleofactor.jpg Lonza-96-Well-Shuttle.jpg

 Nikon dissection microscopes

Nikon-Dissection-Microscope.jpg

TS100 Fluorescent microscope

TS100 Fluorescent microscope.jpg

Countess automated cell counter

Countess-Automated-Cell-Counter.jpg

 Liquid nitrogen and -80 freezer storage space

Liquid-Nitrogen-Freezer-(1).jpg Liquid-Nitrogen-Freezer-(2).jpg _80C-Freezer.jpg

This Core participates in the CHLA Core Pilot Program. To learn more click here.

Working with Human Pluripotent Stem Cells

All research involving human pluripotent stem cells must be reviewed and approved by the Children's Hospital Los Angeles Stem Cell Research Oversight (SCRO) Committee. Investigators requesting H1 or H9 embryonic stem cells will be also be required to sign a Material Transfer Agreement (MTA), termed a simple letter agreement (SLA) from WiCell, before receiving cells. Material Transfer Agreements are required for each cell line used.

For information or questions on SCRO policies and procedures prior to submitting a protocol application, please contact the Institutional Review Board at Children’s Hospital Los Angeles at hspp@chla.usc.edu.

For information and questions on material transfer or to get started on a Material Transfer Agreement, please contact the Office of Technology Commercialization team.

For general lab safety questions and information about lab safety policy, please reach out to labsafety@chla.usc.edu.

Please review the general Core Use Policy.

How to cite the Core in publications:

This work was supported by the Children’s Hospital Los Angeles Stem Cell Analytics Core Facility. The Stem Cell Analytics Core is a CHLA funded shared laboratory.

Following any publication that cites the Core, please inform the Core at stemcellcore@chla.usc.edu

Recent publications include:

Prameela Bharathan S, Ferrario A, Stepanian K, Fernandez GE, Reid MW, Kim JS, Hutchens C, Harutyunyan N, Marks C, Thornton ME, Grubbs BH, Cobrinik D, Aparicio JG, Nagiel ACharacterization and staging of outer plexiform layer development in human retina and retinal organoids._Development. 2021 Dec 1;148(23):dev199551. doi: 10.1242/dev.199551. Epub 2021 Dec 8.PMID: 34738615

Browne A, Arnesano C, Harutyunyan N, Kuu T, Pollack H, Lee TC, Fraser S, Moats R, Aparicio JG, Cobrinik D. Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging. Invest Ophthalmol Vis Sci. 2017 Jul 1;58(9):3311-3318. PMID:28672397

Aparicio JG,  Hopp H, Choi A, J, Liao VC, Harutyunyan N,  Lee TC. Temporal expression of CD184(CXCR4) and CD171(L1CAM) identifies distinct early developmental stages of human retinal ganglion cells in embryonic stem cell derived retina. Exp Eye Res. 2017 Jan 17; 154:177-189. PMID:27867005  

Schlieve CR, Fowler KL, Thornton M, Huang S, Hajjali I, Hou X, Grubbs B, Spence JR, Grikscheit TC.  Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine. Stem Cell Reports. 2017 Sep 12; 9(3):883-896. PMID: 28803915