Stem Cell Analytics Core
Stem Cell Analytics Core
The Stem Cell Analytics Core in The Saban Research Institute at Children's Hospital Los Angeles is a shared lab and centralized support facility funded by the California Institute for Regenerative Medicine. Our main goal is to provide researchers with access to knowledge, resources, equipment and assistance working with pluripotent stem cells.
The Stem Cell Analytics Core and shared lab offers a variety of services focused around 4 key areas:
- Cells and reagents
- Equipment and laboratory space
- Technical assistance
- Research
- Fee Structure
| Cells/Cell Services | Size | Price |
|---|---|---|
| H1, H9, WTC-11 Feeder Free | 1 35mm dish | $40 |
| MEF base cultures available upon request | 1 35mm dish | $40 |
| Each additional dish | $5/dish | |
| Technical support | $35/hr | |
| Freezing cell lines | $10/vial | |
| Maintenance PSC/iPSC cell line provided by researcher | $100/line/week | |
| Equipment | Price | |
| ABI qPCR X2 (96 well,384 well and TLDA) | $15/hr | |
| Biotek Reader | $5/use | |
| Nucleofector | $15/use | |
| Nikon TS100 Fluorescent tissue culture microscope* | $20/hr | |
| *included in regular user's monthly charge or $100/month maximum | ||
| Services | Price | |
| Mycoplasma testing (needed for NIH grants) | $30/sample | |
| STR authentication via University of Arizona (needed for NIH grants) | $66/sample (if submitting genomic DNA)** | $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)** | |
| ** Free shipping to offer to all TSRI once per month | ||
| Facility charges | Price | |
| Facility usage charge (1st hood and all equipment) | $150/month | |
| Facility usage charge (each additional hood) | $75/month | |
| Supplies (Plastic and Media) | At cost | |
| LN2 storage | $5/box/month | |
| Freezer Storage (-20 or -80C) | $5/box/month |
| Cells/Cell Services |
|---|
| H1, H9, WTC-11 Feeder Free | Size: 1 35mm dish | Price $40 |
| MEF base cultures available upon request | Size: 1 35mm dish | Price $40 |
| Each additional dish | Price $5/dish |
| Technical support | Price $35/hr |
| Freezing cell lines | Price $10/vial |
| Maintenance PSC/iPSC cell line provided by researcher | Price $100/line/week |
| Equipment |
| ABI qPCR X2 (96 well,384 well and TLDA) | Price $15/hr |
| Biotek Reader | Price $5/use |
| Nucleofector | Price $15/use |
| Nikon TS100 Fluorescent tissue culture microscope* | Price $20/hr |
| *included in regular user's monthly charge or $100/month maximum |
| Services |
| Mycoplasma testing (needed for NIH grants) | $30/sample |
| STR authentication via University of Arizona (needed for NIH grants) | $66/sample (if submitting genomic DNA)** | $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)** |
| ** Free shipping to offer to all TSRI once per month |
| Facility charges |
| Facility usage charge (1st hood and all equipment) | $150/month |
| Facility usage charge (each additional hood) | $75/month |
| Supplies (Plastic and Media) | At cost |
| LN2 storage | $5/box/month |
| Freezer Storage (-20 or -80C) | $5/box/month |
| Note: Prices are subject to change in the future |
Research Overview
We consider all research projects, including:
- Development of pediatric disease models and building a pediatric iPSC disease bank
- Cell line production
- Differentiation of stem cells to specialized cell types
- Development of methods for genetically modifying stem cells.
Research Services
The Stem Cell Analytics Core offers cell distribution, biobanking, repository and stem cell testing services, assisting both Children's Hospital Los Angeles investigators and non-hospital scientists with their research. Learn more about our Research Services.
Equipment
The Stem Cell Analytics Core facility offers two shared laboratory spaces, imaging equipment, real-time PCR, nuclear transfection, flow cytometry and long term storage. Learn more about our equipment.
Technical Assistance and Learning
The Stem Cell Analytics Core offers technical assistance, protocols, stem cell culture techniques courses and human pluripotent stem cell workshops in The Saban Research Institute at Children's Hospital Los Angeles. Learn more about Technical Assistance.
Ordering Cells and Reagents
The Stem Cell Analytics Core offers a wide range of services for both new and existing clients. Orders are available for conditioned media, reagents, live and frozen cells. Learn more about Stem Cell orders.
Working at the Stem Cell Analytics Core
All investigators at the Stem Cell Analytics Core must meet the general and lab safety requirements. Learn more about working at the Core.
Funding and Billing
The Children's Hospital Los Angeles Stem Cell Analytics Core is funded by the California Institute for Regenerative Medicine. Learn more about funding and billing.
Stem Cell Analytics Core Members
- David Cobrinik, MD, PhD, Core Director
- Jennifer Aparicio, PhD, Core Co-Director
- Narine Harutyunyan
- Andrew Salas