Stem Cell Analytics Core
About Us
The Stem Cell Analytics Core is a centralized support facility for the entire research community whose primary mission is to facilitate stem cell research, innovation and interactions to ensure the success of funded research projects.
How We Support
The Stem Cell Analytics Core provides support by allowing investigators to rent shared, meticulously maintained, and fully equipped tissue culture facilities as well as utilize facility staff for tissue culture support, training, and supplies management (including ordering, inventorying, stocking).
Additionally, the Core provides cell line validation services such as mycoplasma testing and third party cell line authentication as well as access to shared equipment such as a Nucleofector, a fluorescence plate reader, real time PCR machines, fluorescence and dissection microscopes and freezers.
- Research Services and Equipment
- Fee Structure
- Stem Cell Analytics Core Policies
- Publications
The Stem Cell Analytics Core offers cell distribution, biobanking, repository and stem cell testing services, assisting both Children’s Hospital Los Angeles investigators and external investigators with their research.
The staff at the Stem Cell Analytics Core provides high quality human pluripotent stem cell lines (hPSC), hPSC culture training, mycoplasma testing, cell line authentication as well access to rent shared, meticulously maintained and fully equipped tissue culture facilities. Additionally, the Stem Cell Analytics Core can help investigators using the Core with supplies management, including ordering, stocking and keeping inventory, and they can provide tissue culture supplies, like pluripotent stem cell related reagents, at a discount through bulk purchasing.
The following equipment is available within the Stem Cell Analytics Core:
- Amaxa Nucleofector II with or without Lonza 96-well shuttle
- Biotek UV fluorescence plate reader
- Countess automated cell counter
- TS100 Fluorescent microscope
- Nikon dissection microscopes
- Liquid nitrogen and -80 freezer storage space
- 2 ABI 7900 HT fast 96- and 384-well qPCR machines
| Cells/Cell Services | Size | Price |
|---|---|---|
| H1, H9, WTC-11 Feeder Free | 1 35mm dish | $40 |
| MEF base cultures available upon request | 1 35mm dish | $40 |
| Each additional dish | $5/dish | |
| Technical support | $35/hr | |
| Freezing cell lines | $10/vial | |
| Maintenance PSC/iPSC cell line provided by researcher | $100/line/week | |
| Equipment | Price | |
| ABI qPCR X2 (96 well,384 well and TLDA) | $15/hr | |
| Biotek Reader | $5/use | |
| Nucleofector | $15/use | |
| Nikon TS100 Fluorescent tissue culture microscope* | $20/hr | |
| *included in regular user's monthly charge or $100/month maximum | ||
| Services | Price | |
| Mycoplasma testing (needed for NIH grants) | $30/sample | |
| STR authentication via University of Arizona (needed for NIH grants) | $66/sample (if submitting genomic DNA)** | $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)** | |
| ** Free shipping to offer to all TSRI once per month | ||
| Facility charges | Price | |
| Facility usage charge (1st hood and all equipment) | $150/month | |
| Facility usage charge (each additional hood) | $75/month | |
| Supplies (Plastic and Media) | At cost | |
| LN2 storage | $5/box/month | |
| Freezer Storage (-20 or -80C) | $5/box/month |
| Cells/Cell Services |
|---|
| H1, H9, WTC-11 Feeder Free | Size: 1 35mm dish | Price $40 |
| MEF base cultures available upon request | Size: 1 35mm dish | Price $40 |
| Each additional dish | Price $5/dish |
| Technical support | Price $35/hr |
| Freezing cell lines | Price $10/vial |
| Maintenance PSC/iPSC cell line provided by researcher | Price $100/line/week |
| Equipment |
| ABI qPCR X2 (96 well,384 well and TLDA) | Price $15/hr |
| Biotek Reader | Price $5/use |
| Nucleofector | Price $15/use |
| Nikon TS100 Fluorescent tissue culture microscope* | Price $20/hr |
| *included in regular user's monthly charge or $100/month maximum |
| Services |
| Mycoplasma testing (needed for NIH grants) | $30/sample |
| STR authentication via University of Arizona (needed for NIH grants) | $66/sample (if submitting genomic DNA)** | $75.6/sample (if submitting cell pellet, tissue, buccal swab, or blood which require DNA extraction)** |
| ** Free shipping to offer to all TSRI once per month |
| Facility charges |
| Facility usage charge (1st hood and all equipment) | $150/month |
| Facility usage charge (each additional hood) | $75/month |
| Supplies (Plastic and Media) | At cost |
| LN2 storage | $5/box/month |
| Freezer Storage (-20 or -80C) | $5/box/month |
| Note: Prices are subject to change in the future |
Working with Human Pluripotent Stem Cells
All research involving human pluripotent stem cells must be reviewed and approved by the Children's Hospital Los Angeles Stem Cell Research Oversight (SCRO) Committee. Investigators requesting H1 or H9 embryonic stem cells will be also be required to sign a Material Transfer Agreement (MTA), termed a simple letter agreement (SLA) from WiCell, before receiving cells. Material Transfer Agreements are required for each cell line used.
For information or questions on SCRO policies and procedures prior to submitting a protocol application, please contact the Institutional Review Board at Children’s Hospital Los Angeles at hspp@chla.usc.edu.
For information and questions on material transfer or to get started on a Material Transfer Agreement, please contact the Office of Technology Commercialization team.
For general lab safety questions and information about lab safety policy, please reach out to labsafety@chla.usc.edu.
Investigators who received services provided by the Stem Cell Analytics Core are required to acknowledge the Core in publications. The following statement is provided for use in publications:
This work was supported by the Children’s Hospital Los Angeles Stem Cell Analytics Core Facility. The Stem Cell Analytics Core is a CHLA funded shared laboratory.
Following any publication that cites the Core, please inform the Core at stemcellcore@chla.usc.edu
Recent publications include:
Browne A, Arnesano C, Harutyunyan N, Kuu T, Pollack H, Lee TC, Fraser S, Moats R, Aparicio JG, Cobrinik D. Structural and Functional Characterization of Human Stem-Cell-Derived Retinal Organoids by Live Imaging. Invest Ophthalmol Vis Sci. 2017 Jul 1;58(9):3311-3318. PMID:28672397
Aparicio JG, Hopp H, Choi A, J, Liao VC, Harutyunyan N, Lee TC. Temporal expression of CD184(CXCR4) and CD171(L1CAM) identifies distinct early developmental stages of human retinal ganglion cells in embryonic stem cell derived retina. Exp Eye Res. 2017 Jan 17; 154:177-189. PMID:27867005
Schlieve CR, Fowler KL, Thornton M, Huang S, Hajjali I, Hou X, Grubbs B, Spence JR, Grikscheit TC. Neural Crest Cell Implantation Restores Enteric Nervous System Function and Alters the Gastrointestinal Transcriptome in Human Tissue-Engineered Small Intestine. Stem Cell Reports. 2017 Sep 12; 9(3):883-896. PMID: 28803915