Research Seminar: “Spatial genomics and single cell lineage dynamics by seqFISH and MEMOIR”

Speaker: Long Cai, PhD, Research Professor, Department of Biology and Biological Engineering, California Institute of Technology

Talk Summary: Identifying the spatial organization of tissues at cellular resolution from single cell gene expression profiles is essential to understanding many biological systems. We have developed an in situ 3D multiplexed imaging method to quantify hundreds of genes with single cell resolution via Sequential barcoded Fluorescence in situ hybridization (seqFISH) (Lubeck et al., 2014). We used seqFISH to identify unique transcriptional states by quantifying and clustering up to 249 genes in 16,958 cells. By visualizing these clustered cells in situ, we identified regions within distinct composition of cells in different transcriptional states. Together, these results demonstrate the power of seqFISH in transcriptional profiling of complex tissues.  Lastly, I will discuss our work in writing lineages and cell event history into genome of cells by CRISPR/Cas9 genome editing and reading out the stored information in single cells by seqFISH.

Hosted by Pat Levitt, PhD, Simms/Mann Chair in Developmental Neurogenetics, Institute for the Developing Mind, Children's Hospital Los Angeles, WM Keck Provost Professor of Neurogenetics, Keck School of Medicine of USC

RSVP is Required to tecpad@chla.usc.edu