About Us
The CHLA Extracellular Vesicle Core provides the research community with expertise, optimized tools and emerging technologies to support research in the developing fields of Extracellular Vesicle and nanoparticles research. The core provides isolation, characterization, and analysis of extracellular vesicles and other nano-sized particles (e.g. viruses, liposomes, nanobubbles). Established in July 2018, this is the first core in the country dedicated to the rapidly evolving field of Extracellular Vesicle research and applications.
Contact the Extracellular Vesicle Core
4546 Sunset Blvd.
Smith Research Tower, Room 215
Los Angeles, CA 90027
United States
Paolo Neviani, PhD
Technical Director
Phone: 323-361-8564
Email: pneviani@chla.usc.edu
Services
The CHLA Extracellular Vesicle (EV) Core provides the research community with expertise, optimized tools and emerging technologies to support research in the developing fields of Extracellular Vesicle and nanoparticles research.
Provided services:
- EV isolation with a choice of standard and up-to-date methodologies.
- EV analysis by Nanoparticle Tracking Analysis (NTA) with Nanosight NS300 instrument
- EV characterization by
- ExoView R100 platform: based on Single Particle Interferometric Reflectance Imaging Sensor (SP- IRIS) technology
- Classical SDS-PAGE and immunoblotting
- Exosomal RNA analysis by TaqMan-based quantitative real-time PCR
- Generation of EV-depleted FBS.
- User accessible instruments:
- Beckman Ultracentrifuge
- KrosFlo Tangential Flow Filtration syste
Please review the general Core Use Policy.
CoreConnect
This facility utilizes CoreConnect, a web-based core management system that supports the centralization of services and equipment scheduling, billing and usage tracking. Use of the new system is required for all core users, core leaders and core staff.
Learn more about CoreConnect
This Core participates in the CHLA Core Pilot Program. To learn more click here.
CHLA welcomes external users to utilize our Core facilities. Please contact Paolo Neviani, PhD at pneviani@chla.usc.edu or call 323-361-8564.
Access for Non-Hospital Researchers
Access to ECV Core facilities for researchers at USC and other institutions can be arranged. Interested researchers should contact the Technical Director to inquire about availability and rates.