Metabolic Core
The Metabolic Core offers access to state-of-the-art technology and high quality metabolic phenotyping services to basic science researchers studying diabetes, obesity and metabolism. Phenotyping experiments are conducted at CHLA by our highly trained and experienced core personnel using the most up to date, standardized techniques.
Services
- Body composition (NMR)
- Indirect calorimetry
- Locomotor activity
- Food intake
- Glucose and insulin tolerance
- Pyruvate tolerance
- Leptin tolerance
- Glucose CLAMP
- Sample collection
- Hormone and lipid assay
- Core temperature
- Consultation and training
- TissueLyser
For Investigators
For more information about utilizing the Metabolic Lab, please contact Bennie Harris at beharris@chla.usc.edu.
Fee Structure
| Equipment/Service | Fee |
|---|---|
| Body Composition | $15 |
| Metabolic Cages | $800 (first run)- CHLA/USC $400 (second run)- CHLA/USC $1,200 (first run)- External Investigators $800 (second run)- External Investigators |
| Glucose Tolerance | $150/sample |
| Insulin Tolerance | $150/sample |
| Pyruvate Tolerance | $150/sample |
| Hyperinsulinemic, Euglycemic glucose CLAMP | $300/sample- CHLA/USC $400/sample- External Investigators |
| Plasma Hormone Analysis | $100 service fee/plate + the cost of ELISA kit |
| Plasma Lipid Analysis | $10/sample |
| Core Body Temperatures | $5/sample |
| Tissue Collection | $15-$25/sample |
| Equipment/Service |
|---|
| Body Composition | Fee: $15 |
| Metabolic Cages | Fee: $800 (first run)- CHLA/USC | $400 (second run)- CHLA/USC | $1,200 (first run)- External Investigators | $800 (second run)- External Investigators |
| Glucose Tolerance | Fee: $150/sample |
| Insulin Tolerance | Fee: $150/sample |
| Pyruvate Tolerance | Fee: $150/sample |
| Hyperinsulinemic, Euglycemic glucose CLAMP | Fee: $300/sample- CHLA/USC | $400/sample- External Investigators |
| Plasma Hormone Analysis | Fee: $100 service fee/plate + the cost of ELISA kit |
| Plasma Lipid Analysis | Fee: $10/sample |
| Core Body Temperatures | Fee: $5/sample |
| Tissue Collection | Fee: $15-$25/sample |
Core Acknowledgment in Publications
How to cite the Core in publications:
This work was performed with the support of the Metabolic Core of The Saban Research Institute at Children's Hospital Los Angeles.
Recent Publications:
Croizier, S., Park, S., Maillard, J., & Bouret, S. G. (2018). Central Dicer-miR-103/107 controls developmental switch of POMC progenitors into NPY neurons and impacts glucose homeostasis. ELife. 10.7554/eLife.40429
van der Klaauw, A. A., Croizier, S., Mendes de Oliveira, E., Stadler, L. K. J., Park, S., Kong, Y., … Bouret, S.G., Farooqi, I. S. (2019). Human Semaphorin 3 Variants Link Melanocortin Circuit Development and Energy Balance. Cell. https://doi.org/10.1016/j.cell.2018.12.009
Woodward NC, Crow AL, Zhang Y, Epstein S, Hartiala J, Johnson R, Kocalis H, Saffari A, Sankaranarayanan I, Akbari O, Ramanathan G, Araujo JA, Finch CE, Bouret SG, Sioutas C, Morgan TE, Allayee H. Exposure to Nanoscale Particulate Matter from Gestation to Adulthood Impairs Metabolic Homeostasis in Mice. Sci Rep. 2019 Feb 12;9(1):1816. doi: 10.1038/s41598-018-37704-2. PubMed PMID: 30755631; PubMed Central PMCID: PMC6372675.