Stem Cell Core Services

Cell Services

Human Embryonic Stem Cells

The Stem Cell Core maintains the two most widely used lines, H1 and H9, from the ‘H’ family of human embryonic stem cell lines were originally derived by Jamie Thomson at the University of Wisconsin in 1981 (1). These cells are grown in a variety of plates and dishes, on MEF feeders or feeder-free conditions on Geltrex in MEF conditioned media.
1.  Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM. Science. 1998. Vol 282. No 5391. pp 1145-7.

 

H1/H9- on MEF Feeder Layer

ITEM

PRICE CHLA

PRICE NON-CHLA

35mm

$5

$10

60mm

$10

$20

6-WELL PLATE

$30

$60

 

H1/H9- FEEDER FREE CELLS

ITEM

PRICE CHLA

PRICE NON-CHLA

35mm

$10

$20

60mm

$20

$40

6-WELL PLATE

$60

$120

Embryonic Fibroblasts

MEF feeder layers can be obtained from the Children's Hospital Los Angeles Stem Cell Core as irradiated frozen cells or pre-plated feeder layers.

 

FROZEN IRRADIATED MEFS

ITEM

PRICE CHLA

PRICE NON-CHLA

1x106 CELLS

$10

$20

 

LIVE IRRADIATED MEFS

ITEM

PRICE CHLA

PRICE NON-CHLA

1x106 CELLS

$10

$20

 

PREPLATED MEFS

ITEM

PRICE CHLA

PRICE NON-CHLA

35mm

$5

$10

60mm

$10

$20

6- WELL PLATE

$30

$60

12- WELL PLATE

$30

$60

24- WELL PLATE

$30

$60

150mm

$80

$160

 

Media, Reagents and Antibodies

Complete Media

The Children's Hospital Los Angeles Stem Cell Core stocks and distribute high quality, lot tested Knockout™ Serum Replacement by Life Technologies, a serum free formulation commonly used to grow and maintain human and murine pluripotent stem cells. We also offer complete media for maintaining human pluripotent stem cells with and without MEF feeders.

 

COMPLETE MEDIA

ITEM

INFORMATION

VOLUME

PRICE CHLA

PRICE NON-CHLA

hESC/ hiPSC MEDIUM (KSR or FBS)

Contains DMEM F12, L-glut, 20%, KSR, NEAA, 4ng/ml bFGF. Stable for 2 weeks at 4degC.

100mL

$35

$79

MEF-CONDITIONED KSR MEDIUM

hESC medium with KSR conditioned by co-culture with MEF’s for 24 hours. Stable for 2 weeks at 4degC.

50mL

$35

$40

MEF MEDIUM

Contains DMEM, 10% FBS, L-glut. Stable for 2 weeks at 4degC

100mL

$20

$40

 

KSR (500mL)

ITEM

INFORMATION

PRICE CHLA

PRICE NON-CHLA

KSR (Invitrogen)

Serum replacement formulation for mouse and human pluripotent stem cells

$230

$230

 

Other Bioreagents

 

COLLAGENASE

ITEM

INFORMATION

PRICE CHLA

PRICE NON-CHLA

1mg/mL COLLAGENASE SOLUTION (10mL)

Type IV Collagenase powder from GIBCO diluted in DMEM-F12 basal medium and sterile filtered.  Concentration= 1mg/mL

$10

$15

GELTREXTM (200uL)

Life Technologies Reduced Factor Basement Membrane Matrix for hESC culture

$5

$10

LIF (10ug)

LIF from millipore cat# LIF1010  
http://www.millipore.com/

$221

$221

 

Antibodies

 

GCTM2

DESCRIPTION

High MW cell surface glycoprotein present on undifferentiated stem cells.  Lost during differentiation

ISOTYPE

MOUSE IgM

SPECIES REACTIVITY

HUMAN

RECOMMENDED DILUTION

1:2 to 1:3

APPLICATIONS

FLOW CYTOMETRY, ICC, IHC

QUANTITY

5mL

PRICE

$5

 

TG30

DESCRIPTION

CD9, a cell surface protein known to function during cell development, growth and motility. CD9 is down regulated by differentiating hPSC’s.

ISOTYPE

MOUSE IgG2a

SPECIES REACTIVITY

HUMAN

RECOMMENDED DILUTION

NEAT TO 1:2

APPLICATIONS

FLOW CYTOMETRY, ICC, IHC

QUANTITY

5mL

PRICE

$5

 

Millipore Antibodies- STEM CELL MARKERS

Item

Catalog Number

Amount

Price CHLA

 Price Non-CHLA

 SSEA-1

MAB4301

100ug

$263

$263

SSEA-3

MAB4303

100ug

$263

$263

SSEA-4

MAB4304

100ug

$263

$263

TRA-1-81

MAB4381

100ug

$263

$263

TRA-2-49

MAB4349

100ug

$263

$263

SOX-2

MAB4343

100ug

$263

$263

 

Bioreagent Cabinets

The Stem Cell Core houses two bioreagent cabinets within the facility:

USC Bioreagents Core

The stem cell core also houses a bioreagents refrigerator through the Bioreagents Core at the USC Norris Cancer Center. This refrigerator supplies Children's Hospital Los Angeles with basal media at a discounted rate.

Roche Cabinet

The USC Norris Cancer center also offers a Roche cabinet supplying enzymes and molecular reagents to the researchers on the Children's Hospital Los Angeles campus. Please contact the core for a current list of products and prices. Access to cabinets is available Monday through Friday, 10 a.m. to 4:30 p.m. 

Life Technologies Cabinet

Coming soon! The Stem Cell Core will be housing a Life Technologies Bioreagents Cabinet.

 

Cell Culturing Services

Biobanking

The Stem Cell Core offers biobanking services, in which the core technicians will expand, freeze, thaw and recover human pluripotent stem cell lines investigators bring to the core.

 

hPSC’s BIOBANKING SERVICES

ITEM

DESCRIPTION

PRICE CHLA

PRICE NON-CHLA

HPSC SLOW FREEZING

Cost per 3 vial of cells frozen.

$100

$200

HPSC THAW ONLY

Cost of thawing a vial of hPSC ‘s

$50

$100

HPSC THAW AND EXPANSION

Cost of thawing a vial of hPSC and expanding to generate 2 x 35mm dish

$150

$300

COMMERCIAL HPSC LINE RECOVERY

Cost associated with thawing, expanding and rebanking a hPSC line that is not normally carried by the core to generate 5-10 cryovials.

$500

$1000

OTHER CELL LINES

Contact core manager for a quote. Price will be based on technical assistance fees ($50 per hour)

 

Repository

The Stem cell core offers cell repository services, where researchers can purchase hPSCs and tumor lines being used and stored in the core. The costs for these services are below.

 

CELL LINE REPOSITORY

ITEM

DESCRIPTION

PRICE CHLA

PRICE NON-CHLA

TUMOR LINE

Cost per vial of cells frozen.

$30

$60

TUMOR LINE (RECOVERED CELLS)

Cost per vial of recovered cell

$50

$100

HPSC LINE

Cost for a vial of cells in the repository which are not kept in culture

$100

$200

HPSC LINE, THAWED AND EXPANDED

Cost of thawing a vial of hPSC expanding to generate 3 x 35mm dish

$150

$300

 

Cell Expansion

The Core will expand hPSCs, as well as expand and bank adult stem cell lines and other cell lines as requested.

 

CELL LINE EXPANSION

ITEM

PRICE CHLA

PRICE NON-CHLA

EXPANSION AND BANKING OF CELL LINES IN T-FLASKS

$50

$100

EXPANSION OF HPSCS

$100

$200

 

Mycoplasma Testing

Mycoplasma testing is available as requested. Testing is performed using the Lonza MycoAlert testing kit.

Please note, mycoplasma testing is required prior to introducing new cell lines into Shared Facility, either through the core or individually through RADIL (or equivalent) with proof of negative results.

 

MYCOPLASMA TESTING

ITEM

PRICE CHLA

PRICE NON-CHLA

MYCOPLASMA TESTING (PER SAMPLE)

$30

$60

 

Current ServicesStem Cell Core Research Services

Stem Cell Core Order Forms

Please email the Stem Cell Core with the completed forms.

Future Services

  • Additional hESC lines
  • iPSC derivation
  • Other cells lines as requested
  • Stem Cell Specific bioreagents cabinet