Small Animal Imaging Core

The Small Animal Imaging Core provides investigators with access to radiology imaging equipment and specialized high end computers and software for research that involves animal models of health and disease. Its function is similar to radiology departments in the clinical setting. Staffed by highly experienced research imaging specialists and technologists, the core provides images to investigators and facilitates image processing and interpretation via collaboration with the Translational Biomedical Imaging Laboratory, the Optical Imaging Core and individual research laboratories.  The equipment is typical of instrumentation found radiology department with the addition of special optical based techniques useful for imaging very small subjects: Bioluminescence/Fluorescence imaging, Micro-CT, 7 Tesla small bore MRI, High-Res plain film X-ray, Micro-Spect-CT.

The facility is designed to provide not only imaging technologies, but also guidance in terms of imaging protocols. One of the core's major capabilities is handling different kinds of research projects including working with small rodents with or without imaging equipment. The core is open to academic investigators and commercial/non-commercial organizations.

 

 

 

 

 

Services

  • Available imaging scanners
  • Full handling of projects
  • Precise intracranial xenographts
  • Fixed and non-fixed biological sample harvesting
  • Complicated perfusions
  • Imaging data collection, reconstruction, storage
  • Imaging data processing and analyze with different software's-Analyze, Amira, Imaris,  Arivis, Image J, MatLab, Osirix, LightWave, CT-An, Adobe, IVIS Living Image
  • Technical training, personal trainings on imaging modalities, software training
  • Research imaging project creation and consultation
  • Powerful PCs equipped with up to 1TB of RAM

Equipment and Software

CHLA-Research-Imaging-2268-275.jpg7.05T Pharmascan uMRI (Bruker) 2003 - This is a small bore, single channel uMRI , capable of running anatomical studies in  small rodents( up to small rabbits). We generate data according to the studies for tumor development, metastasis, tracking of contrast enhanced cells (tumors, stem cells, etc.). Equipment is capable to run some spectrometries for organic and non-organic compounds. The best in-plane resolution is 83um; in-slice resolution is 320-350 um.

 

CHLA-Research-Imaging-2264-275.jpgIVIS 200 Bioluminescence/Fluorescence (Perkin-Elmer, former Xenogen) 2013 - in vivo imaging technology platform, that allows non-invasive visualization and tracking of cellular and genetic activity within a living organism in real time.  This equipment is work-horse with high throughput capacity. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using Digital Mouse Atlas.

 

 

 

 

 

 

 

Faxitron MXCHLA-Research-Imaging-2265-275.jpg-20- is the industry standard in cabinet X-ray systems for point-of-care specimen/small rodent radiography, specified in bone studies and implants.

 

 

 

 

CHLA-Research-Imaging-2266-275.jpgSkyScan1172 HighRes uCT (Bruker) - High-resolution desktop X-ray  uCT system for small samples. The flexible geometry of the scanner is particularly advantageous over intermediate resolution levels.  Good for biological tissue structural studies.

 

 

 

 

CHLA-Research-Imaging-2267-275.jpgSPECT/CT - This high resolution SPECT system has numerous advantages over the use of clinical gamma cameras and micro-PET systems, including:  CT and SPECT to form fusion images to localize radioisotopes signal.

 

 

 

 

Fee Structure

The Research Imaging core provides basic images to the investigator. Image processing and interpretation require collaboration with the Radiology Department. 

  • uMRI- $200/hr
  • uCT- $150/hr
  • BLI- $130/hr
  • X-ray-$10/scan
  • CT/SPECT- $150 (doesn’t include radioisotope)
  • Intracranial xenographts/lung perfusion( survival and non-survival  surgeries)- $50/sample
  • Animal handling, treatments, biological tissue harvesting -$25/hr
  • Data reconstruction- $40/hr
  • Data processing and analyze- $80/hr
  • Imaging consultation- $100/hr
  • Software training-$80/hr
  • Full project handling
  • Publications
  • Clients
  • Imaging Data Analysis

Sims JD, Hwang JY, Wagner S, Alonso-Valenteen F, Hanson C, Taguiam JM, Polo R, Harutyunyan I, Karapetyan G, Sorasaenee K, Ibrahim A, Marban E, Moats R, Gray  HB, Gross Z, Medina-Kauwe LK. A corrole nanobiologic elicits tissue-activated MRI contrast enhancement and tumor-targeted toxicity. J Control Release. 2015 Nov 10;217:92-101. doi: 10.1016/j.jconrel.2015.08.046. Epub 2015 Aug 31. PubMed PMID: 26334483; PubMed Central PMCID: PMC4623877.

Bonne NX, Vitte J, Chareyre F, Karapetyan G, Khankaldyyan V, Tanaka K, Moats  RA, Giovannini M. An allograft mouse model for the study of hearing loss secondary to vestibular schwannoma growth. J Neurooncol. 2016 Aug;129(1):47-56. doi: 10.1007/s11060-016-2150-9. Epub 2016 May 13. PubMed PMID: 27177628.

Morshed RA, Gutova M, Juliano J, Barish ME, Hawkins-Daarud A, Oganesyan D, Vazgen K, Yang T, Annala A, Ahmed AU, Aboody KS, Swanson KR, Moats RA, Lesniak MS. Analysis of glioblastoma tumor coverage by oncolytic virus-loaded neural stem cells using MRI-based tracking and histological reconstruction. Cancer Gene Ther. 2015 Jan;22(1):55-61. doi: 10.1038/cgt.2014.72. Epub 2014 Dec 19. PubMed PMID: 25525033; PubMed Central PMCID: PMC4293243.

Gutova M, Frank JA, D'Apuzzo M, Khankaldyyan V, Gilchrist MM, Annala AJ, Metz MZ, Abramyants Y, Herrmann KA, Ghoda LY, Najbauer J, Brown CE, Blanchard MS,Lesniak MS, Kim SU, Barish ME, Aboody KS, Moats RA. Magnetic resonance imaging tracking of ferumoxytol-labeled human neural stem cells: studies leading to clinical use. Stem Cells Transl Med. 2013 Oct;2(10):766-75. doi: 10.5966/sctm.2013-0049. Epub 2013 Sep 6. PubMed PMID: 24014682; PubMed Central PMCID: PMC3785261.

Tang Y, Sharma P, Nelson MD, Simerly R, Moats RA. Automatic abdominal fat assessment in obese mice using a segmental shape model. J Magn Reson Imaging. 2011 Oct;34(4):866-73. doi: 10.1002/jmri.22690. Epub 2011 Jul 18. PubMed PMID: 21769982.

Tang Y, Lee S, Nelson MD Jr, Richard S, Moats RA. Adipose segmentation in small animals at 7T: a preliminary study. BMC Genomics. 2010 Dec 1;11 Suppl 3:S9. doi: 10.1186/1471-2164-11-S3-S9. PubMed PMID: 21143791; PubMed Central PMCID: PMC2999354.

University of California, San Diego

University of California, Los Angeles

University of Southern California

Children’s Hospital Los Angeles

City of Hope

Cedars-Sinai Medical Center

GAMMAMEDICA

House Research Institute

ALLERGAN

Tocagen

The Research Imaging Core is equipped with a wide variety of commercial tool sets for imaging data analysis and visualization.

CTAn, CTVol - Bruker's data analysis and visualization tool suite

Analysis - Olympus's image analysis software

Amira - FEI's platform for 3D and 4D data visualization, processing and analysis

Fiji (ImageJ) - A NIH image processing tool

Lightwave 3D - NewTek's 3D computer graphics software

Imaris - 3D and 4D Real-Time Interactive Data Visualization Platform

Living image - In Vivo Imaging Software

Living image - In Vivo Imaging Software