Vector Design and Construction
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Basic Cloning
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$500
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Basic Cloning w/Sequencing
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$500 + $50 = $550
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Basic Cloning w/PCR + 2 primer sequencing*
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$500 + $50 = $550
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Intermediate Cloning w/Sequencing
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$900 + $50 = $950
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Intermediate Cloning w/PCR*
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$900 + $50 = $950
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Custom Cloning
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Price available by quote
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*For genes larger than 600bp, add $125 for every additional 200bp.
Vector Packaging
We charge $1500 and $2500 for 2L and 4L preparations and concentration by tangential flow filtration, with a yield of 1-5 x 10^9 virus particles/mL in a final volume of 5-10 mL, respectively. For an additional fee, we can concentrate the virus again over a second column to a final volume of 1.5-3 mL.
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Endotoxin-free maxiprep
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$400 per 3
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Concentrated by Tangential Flow Filtration: 1-5 x 109 virions/mL
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Packaging retroviral vector
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$1,900
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Packaging retroviral vector lacking selectable marker 2L
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$2,200
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Packaging of lentiviral vector, 2L
Packaging of lentiviral vector, 4L
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$1,500
$2,500
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Packaging lentiviral vector lacking selectable marker, 2L
Packaging lentiviral vector lacking selectable marker, 4L
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$1,800
$2,800
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Not concentrated: 108 virions
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Packaging retroviral vector
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$800
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Packaging retroviral vector lacking selectable marker
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$1,000
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Packaging of lentiviral vector
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$500
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Packaging lentiviral vector lacking selectable marker
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$700
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Ready-made lentivirus with tracking gene (F-luciferase, R- luciferase, eGFP, mCherry, tdTomato)
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Concentrated: 109 virions/mL
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$1,500
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Not concentrated108 virions
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$500
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Replication competent assay, titering by FACS and drug selection are included in the pricing above.
Titering by qPCR in the absence of selectable or fluorescent marker is NOT including in the pricing above.
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