The Vector Core is now open for IBC approved investigators to use the facility. We anticipate being fully functional by July 2014. We are also able to provide intellectual assistance at this point. Please note, all cell lines must be certified as mycoplasma negative prior to use in the facility.
About the Vector Core
The Vector Core provides assistance in the design and construction viral vectors, as well as in the production of viral particles for gene-modified in vitro as well as in vivo studies. We currently can help design or produce vectors for experiments involving retrovirus, lentivirus, HSV-1, and AAV. We have numerous viral vectors that can be used by the Children’s Hospital Los Angeles and USC communities. The Vector Core also provides consulting services to help researchers identify their gene transfer needs, determine the best way to produce gene-modified cells, and to assist in experimental troubleshooting. To this end, members of the Vector Core provide a broad range of core competencies that will benefit our hospital researchers.
Vector Core Members
Vector Core Director, Shelley Hough
Faculty Director, Rusty Lansford, PhD
The Vector Core offers a wide range of services, some of which are tailored to the specific needs of the client. As a result, we find it beneficial for new clients to undergo an introductory consultation with the manager prior to the onset of regular service.
Equipment and Shared Space
Please contact Shelley Hough or Rusty Lansford, MD for information about the lab and to become a registered user of the equipment and space. An approved IBC from your institution is required to work in the Vector Core or to receive active viruses from the Vector Core.
COLLECTION OF COMPLETE MEDIA
Perishable reagents such as complete media may be collected once email conformation of the order is received. Media must be collected within one week of receiving email conformation. Uncollected material will be discarded and charged for at the regular rate.
Frozen cells, viral vectors and virus may be collected once email conformation of the order is received. These items must be collected within one month of receiving email conformation. Orders that are not collected within the one month holding period will be cancelled and subject to a $10 storage fee.
All investigators at the Vector Core must meet general and lab safety requirements.
Please read and understand the following:
Acknowledging the Vector Core
Clients receiving services from the Children's Hospital Los Angeles Vector Core are required to acknowledge the facility according to the posted instructions on the Acknowledging the Core web page. The following text is provided for use in publications:
All investigators must have up-to-date lab safety and bio hazardous material training and permission from their Principal Investigator.
All investigators must have prior cell culture experience and up to date blood borne pathogen training in order to work with living cells. We also recommend individuals working with human cells undergo vaccination for Hepatitis B. Training in cell culture techniques can be arranged by contacting Laura-Marie Nucho in the Stem Cell Core.
Regulatory Requirements and Transfer Forms
For questions on the MTA submission process contact Jessica Rousset in the Children’s Hospital Los Angeles Technology Transfer office.
Weekend and Holiday Work
The shared labs at the Vector Core facility will remain open to investigators throughout weekends and all national holidays. Investigators are responsible for tending their own cells and experiments during holidays and weekends.
Please note that the Vector Core staff will not be available during holidays or weekends to provide cells, reagents or technical assistance. It is the responsibility of the investigator to place and collect orders in a timely manner and to ensure they have sufficient cells and reagents for the vacation period.
Charges for supplies, cells, equipment usage, consultation and technical assistance are billed monthly. Billing is compiled by the Vector Core manager and invoices are issued by the Children's Hospital Los Angeles Research Operations and Facilities Department by email at the end of each month.
Children's Hospital Los Angeles Clients
Please provide a grant number that the materials can be billed to. Charges are compiled at the end of the month and sent to the Research Operations and Facilities Department. An invoice will be generated and emailed to the Principal Investigator for review prior to the grant being charged.
Non-Children's Hospital Los Angeles Clients
Charges are compiled at the end of the month and sent to the Children's Hospital Los Angeles financial department. An invoice will be generated, emailed to the Principal Investigator and a check sent to the Research Operations and Facilities Department.