Cellular Imaging Equipment and Software
All microscopes are equipped with high-resolution objective lenses and digital cameras to produce the highest quality images and time-lapse movies.
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Microscopes
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Scanners
Image Processing Workstations & Software
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Zeiss LSM 710 Inverted Confocal Microscope (Saban 338)
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- Specializes in confocal and three-dimensional fluorescence imaging
- Acquisition of up to 3 fluorescence channels simultaneously
- Laser lines: 405, 458, 488, 514, 561, and 633 nm
- DIC/Nomarski imaging simultaneous with fluorescence
- Capable of advanced analytical techniques like FRAP, FRET, and spectral unmixing
- 35mm Petri dish warmer available for live cell imaging
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Leica DM RXA Upright Fluorescence Microscope (Smith 1030)
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- Specializes in widefield fluorescence imaging
- Fluorescence excitation with xenon lamp for even, stable illumination
- DIC/Nomarski and phase-contrast imaging
- Spectral imaging
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Leica MZFLIII Stereo Microscope (Saban 439)
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- Specializes in imaging of embryos and other whole specimens
- Fluorescence
- Reflected and transmitted light
- Motorized focus (z) drive for “extended depth of focus” images
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Zeiss Axioplan Upright Microscope
(Smith 1030)
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- Specializes in color brightfield imaging
- Color CCD camera
- Also capable of standard blue, green, and red fluorescence.
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Zeiss Axio Observer Inverted Confocal, Epifluorescence, & Color Transmitted Light Microscope (Saban 448)
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- Versatile microscope that can image virtually any specimen on a slide or culture dish
- LSM 700 confocal scanner with laser lines of 405, 488, 555, and 639 nm
- Color CCD camera for histology and immunohistochemistry slides
- Sensitive high-resolution monochrome camera for epifluroescence
- ApoTome structured illumination system
- Capable of advanced analytical techniques like FRAP, FRET, and spectral unmixing
- DIC/Nomarski, and phase contrast
- Motorized stage for tiling/stitching large areas
- 35mm Petri dish warmer available for live cell imaging
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CARV II Spinning Disk Live Cell Imaging Confocal (Smith Tower 322C)
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- Nipkow spinning disk confocal system with lamp-based excitation (no lasers) for gentler confocal imaging of live cells
- Enclosed in environmental chamber to control temperature, CO2, and O2 for imaging live cells over extended time
- Capable of standard blue, green, red, and far red fluorescence in confocal or widefield mode
- DIC/Nomarski, and phase contrast
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Pathscan Enabler Slide Scanner (Smith 1030)
- Specializes in imaging serial or large sections on slides
- Scans a standard microscope slide in 5 minutes or less
- Resolution equivalent to 5x microscope objective
Image Processing Workstations & Software
(Saban 309 and Smith Tower 1030C)
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- MetaMorph – powerful 2D and 3D image processing and analysis including morphometry and movie making
- AutoDeblur – for deconvolution of confocal images to reduce noise and increase resolution
- Photoshop – image preparation for publication and presentation
- FoveaPro – scientific image processing and analysis add-ons for Photoshop
- ImageJ – versatile, customizable, open-source scientific image processing and analysis software. The core’s Imaging Scientist can write custom software for ImageJ to suit your needs
- MATLAB Image Processing Toolbox - fast, powerful programming environment for image processing
Locations:
The Saban Research Institute
4661 Sunset Blvd.
Los Angeles, CA 90027
Smith Research Tower
4546 Sunset Blvd.
Los Angeles, CA 90027
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