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Fluorescence Activated Cell Sorting

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The Fluorescence Activated Cell Sorting (FACS) core provides researchers with a powerful method to study and purify cells for wide applications in immunology, cell biology and other fields of biology.

Flow Cytometry is a technology used to make fluorescent measurements of special fluorochromes bound to or made by various cells as they travel in single file through one or more laser lines while confined by a laminar fluid. Common applications involve immunolabeling of extracellular or intracellular proteins with fluorochrome-conjugated antibodies, reactive dyes, or nucleic acid dyes. Fluorochromes are excited to emit various colors of light by the lasers. Data acquisition is achieved with specialized computer software directly interfaced with the cytometer.

Services

  • Absolute Counts
  • Apoptosis
  • Calcium Flux
  • Co-localization*
  • Cytometic Bead Assay (CBA) for up to 30 soluble cytokines or proteins per sample
  • DNA Cell Cycle Analysis
  • Flow-FISH for telomere length
  • Immunophenotyping for Surface, Intracellular and Nuclear proteins
  • Live cell sorting, PhosFlow
  • Luminex multiplex assays
  • Rare Event Detection/Sorting
  • Reporter Molecules
  • Side Population
  • Single-cell Cloning
  • Single Color Apoptosis*
  • Sizing
  • Spot Counting for viral load*, Subcellular compartmentalization*, Synapse activity*
  • Translocation*
  • Viability
* denotes applications specific to the Imagestream 100
 
 

Hours of Operation

Schedule for Sorting Assistance and Trained User Analyzer

2nd Floor FACS
9 a.m. - 5 p.m.

3rd Floor FACS
10 a.m. - 7 p.m.
*  For after-hours access for 2nd floor Trained User Analyzer, call (323) 361-2313.
Available 24 hours a day, 7 days a week. 
 


Contact the FACS Core

Collaborations | Consultations  |  Service Issues
Non-Standard Applications

Appointments | Training Questions

Hisham Abdel-Azim, M.D., Director, 2nd Floor
e-Mail | Phone:  (323) 361-5664

Michael Sheard, Ph.D., Director, 3rd Floor
e-Mail  |  Phone: (323) 361-2297

Ann George, Sort Operator, 2nd Floor
e-Mail  |  Phone: (323) 361-5935

Ewa Zielinska, Sort Operator, 2nd Floor
e-Mail | Phone: (323) 361-5953

Jackie Lin, Sort Operator, 3rd Floor
e-Mail | Phone: (323) 5455

Location
Smith Research Tower
4546 Sunset Blvd.
2nd Floor: Rooms 203 and 205, MS#62
3rd Floor: Room 301, MS #57
Los Angeles, CA 90027 

Parking
There is visitor parking in the hospital lot across from Smith Research Tower.